Bioinformatics is an interdisciplinary field that develops and improves on methods for storing, retrieving, organising and analysing biological data. A major activity in bioinformatics is to develop software tools to comprehend complex biological systems.
- Differential Gene Expression – Statistical analysis of differential gene expression from transcriptomics data (microarray, RNAseq)
- Variant Identification – Identification of genetic variants (SNPs and InDels) from large scale genomics data
- Methylation and ChIPseq – Exploration of epigenetics and post-transcriptional modifications of the genome and its expression
- De-novo assembly – In silico assembly of transcriptomics or genomics data from non-model organisms
The platform also provides access to the IPA (Ingenuity Pathway Analysis) and Metacore (GeneGo) software via a hot desk for its collaborators.
Visit the Monash Bioinformatics webpage for more information https://platforms.monash.edu/
The Cell Therapies Platform hosts a biological Isolator that provides a sterile, fully contained environment suitable for the processing of human cells and tissue. This facility has been established to boost the clinical development of human cell-based therapies.
The Cell Therapies Platform provides a flexible, cost-effective alternative to traditional cell therapy manufacturing and regenerative medicine facilities. Available to researchers and commercial entities from within and out with the MHTP seeking to progress cell and tissue based therapies to the clinic. The platform houses the BioSpherix Xvivo GMP Isolator, an enclosed, integrated and fully controlled environment for cell and tissue processing. The Miltenyi MACSQuant Tyto clinical cell sorter and the GeSim BioScaffolder with 3D biological printing and melt electrospinning capabilities.
Prof Graham Jenkin
Hudson Institute Department of Obstetrics and Gynaecology, Monash University
t: +61 3 8572 2801
Dr Gordon McPhee
Cell Therapies Platform Manager
MHTP Translational Research Facility
t: +61 3 8572 2802
The Flow Cytometry Facility uses sophisticated instruments for cellular and particle analysis based upon the use of fluorescent probes and markers. Flow cytometers can be used for investigating cellular makeup, enabling detailed analysis of biological activity and function and for isolating rare cell populations for further studies. The facility currently operates one high speed sorter and two cell analysers and provides the following services on a fee-for-service model:
- Cell sorting
- Cell analysis
- Training for independent use of cell analysers
- Advice on experimental design and data analysis
- Dr James Ngui, Senior Scientist, Monash Pathology, Ph. + 61 3 9594 3611, firstname.lastname@example.org
- Ms Jeanne Butler, Research Scientist, Monash University
Based at the Monash Health Translation Precinct, the Functional Genomics Capability provides the tools for unbiased, systematic, high-throughput, genome-wide, gain-of-function and loss-of-function studies in human and mouse cells.
We offer pooled library screens, using CRISPR-Cas9 technology (CRISPRc, CRISPRi, CRISPRa) and ORF libraries, to support investigations ranging from fundamental biology through to addressing clinical need.
We work closely with investigators and deliver comprehensive project management, based upon fee-for-service model, including
- Project design and implementation strategy
- Genome wide or smaller custom designed pooled sgRNA libraries
- Creation of Cas9/dCas9 cells for CRISPR screens
- Pooled library screening and selection
- Massively parallel sequencing (next generation sequencing)
- Bioinformatic analysis and data visualization
- Secondary screening, for example single cell transcriptomics (Dolomite Bio Single Cell RNA-Seq)
- Bioinformatic analysis and mechanistic characterization of secondary screening hits.
A/Prof Joseph (Sefi) Rosenbluh,
MHTP Functional Genomics Capability Director
t: +61 3 9902 9257
Dr Catherine Itman,
MHTP Functional Genomics Capability Manager
t: +61 3 8572 5032
The Hudson Institute's Histology Facility was established in 1999 to support the needs of researchers and students at the Institute. The Facility also provides services for the Monash Health Translation Precinct (MHTP) and external clients. Our aim is to provide high quality, reliable and cost effective histological serviceHistology involves the study of the microscopic anatomy of cells and tissues. Tissues are carefully processed, sectioned and stained revealing delicate morphological features that can indicate health or disease for research, education or clinical studies. The Histology Platform has two service models:
- a full “Professional” end-to-end service provided by specialist staff
- a "Do it Yourself" service providing access to laboratory facilities, specialist equipment and consumables.
In addition, the experienced Histology team can provide advice, training and research assistance in:
- Processing – to resin or paraffin
- Cutting – fresh, resin or paraffin sections
- Staining and coverslipping -automated or manual
- Immunohistochemistry – automated utilising the Dako Autostainer
- Micro Tissue array slide supply – creation of micro tissue arrays to suit your needs. We can create the multi block for you and provide sections for use as control tissue
- Slide scanning
- Digital photography, basic fluorescence and polarization
- Ms Camilla Cohen, Monash Histology Platform Manager, Monash University, Ph. + 61 3 9905 2740, email@example.com
- Ms Angela Vais, MHP-MHTP Histology Node Senior Officer, Hudson Institute of Medical Research, Ph. +61 3 9594 5618, firstname.lastname@example.org, email@example.com
The Mass Spectrometry Facility houses cutting-edge mass spectrometry and proteomics technologies for the study of protein structure and function, and their relationship to human disease. The facility can provide:
- Based upon a collaborative model:
- Consultation to researchers interested in or engaged in biomarker identification, including basic protein identification, fractionation and analysis
- Proteomic analyses for discovery studies, using in-gel and gel-free technologies
- Design and development of Mass spectrometry-based research diagnostics and/or ELISA assays
- Based upon a fee-for-service model:
- A range of NATA certified assays for steroids, metanephrines, vitamins and drugs
- Assay service for research groups
The Medical Genomics Facility provides a comprehensive range of technologies to analyse DNA and RNA, determining its structure and function and its role in health and disease.
We provide a broad range of specialist genomic services based upon a fee-for-service model including
- Sanger Sequencing (ISO 15189 NATA accredited)
- Quantitative real-time PCR
- Fragment Analysis
- Next Generation Sequencing
- Fluidigm single cell genomics
- Cell line identification
- Drop-Seq Technologies
- Vivien Vasic, MHTP Medical Genomics Facility Manager, t: +61 3 8572 2770, e: firstname.lastname@example.org
- Dr Trevor Wilson, Scientific Manager, t: +61 3 8572 2818, e: email@example.com
- Jodee Gould, Microarray Manager, NGS Specialist, t: +61 3 8572 2462, e: firstname.lastname@example.org
Visit the Medical Genomics Facility Centres
MMI charges an annual subscription fee to cover training and instrument access for internal users.
Micro Imaging provides:Micro imaging
- Experimental design and troubleshooting
- Research collaboration
- Project development.
Dr Kirstin Elgass
MMI-MHTP Node Manager
t: +61 3 8572 2790
The Monash Biobank is a recent initiative established by Monash Health’s Pathology department to support translational research through procurement of human samples.
- Assistance with HREC application
- Sample collection and processing following SOP or researcher’s protocol (for prospective and specific studies)
- Sample storage and data collection
- Archived tissue retrieval and selection
- Sectioning and Tissue Micro Arrays to suit project requirements
- Digital imaging
- Dr. Beena Kumar, Unit Head, Department of Anatomical Pathology, Monash Health, Ph. +61 3 9594 3459, Beena.Kumar@monashhealth.org